Best multispectral to RGB conversion

[Jean-Patrick Pommier]

Thank you

Jean-Pat

2012/3/22 Zachary Pincus <zachary.pincus at yale.edu>

> > The steps should be for a given image (ex s-aqua):
> >   *calculate saturation and brightness from gray scale s-aqua. (s1,b1)
> >   *choose hue (a1)
> > Do the same for each fluo
> > Use the 5x3 hsb values to calculate a matrix to do the convertion 5
> channels->3 RGB
> >
> > Is it correct?
>
> Basically correct.
>
> You could also just calculate brightness from the gray images, and leave
> the saturation constant.
>
> Also, my suggestion was to use the "screen" operator to combine each
> image. The 5x3 matrix multiplication effectively makes 5 RGB images (one
> from each fluor), and then sums them together. In general, I find that
> "screen" works better than "sum" for making visually-appealing overlays of
> fluorescence channels.
>
> For [0,1]-valued float images, the formula for screen-combining two images
> a and b is: a+b-ab. (http://en.wikipedia.org/wiki/Blend_modes#Screen )
>
> Anyway, I'd try both sum and screen to see which looks best.
>
> Zach
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mail.python.org/pipermail/scikit-image/attachments/20120323/d2a9e863/attachment.html>