Best multispectral to RGB conversion

[jip]

Each image is a raw image of a given fluorochrome.

A given fluorochrome targets a subset of chromosomes, more informations can 
be found in:

*Automatic Segmentation and Classification of
Multiplex-Fluorescence In-Situ Hybridization
Chromosome Images
by
Hyo Hun Choi, B.S., M.S.
*
Choi gives an example of CCD integration times:
*
An example of integration times is [DAPI, Aqua,
Green, Gold, Red, Far Red] = [0.14, 6, 0.76, 6, 2.96, 1.4] seconds*

He also gives an example of spectral mixing in the raw images:

<https://lh3.googleusercontent.com/-dw-YEQxPyR4/T2r66g-u0EI/AAAAAAAAAvo/NLfMvYuL2Sg/s1600/Vysis.png>
With the vysis data set 
(example:https://github.com/jeanpat/MFISH/tree/master/MFISH_TIFF/V13__40), 
a given fluorochrome corresponds to several chromosomes, in the 
spectrumAqua  image the chromosomes 3,10,11,13,15,20,21,22,X,Y are visibles.

A: S. Aqua
D DAPI
F: Far Red
G: S. Green
R: S. Red
Y: S. Gold

To perform "perfect" spectral unmixing, I suppose it's necessary to know 
the integration time, the characteristic of the microscope bandpass 
filters, the emission spectrum of each fluorochrome; but would it be 
possible to make some kind of "estimated" unmixing from only the images 
themselves?

Best regards
Jean-Pat

Le mercredi 21 mars 2012 12:10:16 UTC+1, jip a écrit :
>
> Dear all,
> Some one asked me how I choose the matrix coef to convert a five 
> multispectral image to a three (RGB) multispectral image<http://dip4fish.blogspot.fr/2011/05/making-mfish-image.html?showComment=1332325696284#c6729349186645008476>. 
> I feel that there is a best way to do that by maximising some distance in a 
> color space.
> How would you do that?
> Examples of multispectral images are available here<https://github.com/jeanpat/MFISH>
>
> Best Regard
>
> Jean-Patrick Pommier
>

Le mercredi 21 mars 2012 12:10:16 UTC+1, jip a écrit :
>
> Dear all,
> Some one asked me how I choose the matrix coef to convert a five 
> multispectral image to a three (RGB) multispectral image<http://dip4fish.blogspot.fr/2011/05/making-mfish-image.html?showComment=1332325696284#c6729349186645008476>. 
> I feel that there is a best way to do that by maximising some distance in a 
> color space.
> How would you do that?
> Examples of multispectral images are available here<https://github.com/jeanpat/MFISH>
>
> Best Regard
>
> Jean-Patrick Pommier
>
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