Here is my code ; why after the readline the port close? ser = serial.Serial(0) ser.baudrate = 9600 ser.parity = serial.PARITY_ODD ser.stopbits = serial.STOPBITS_TWO ser.bytesize =serial.EIGHTBITS ser.setRTS(level = 0) ser.setDTR(level = 0) ser.timeout = 1 ser.readline() Why after this the port close. Regards Luca